Correlative Fluorescence and Electron Microscopy

Simultaneous Correlative Scanning Electron and High-NA Fluorescence Microscopy

Correlative light and electron microscopy (CLEM) is a unique method for investigating biological structure-function relations. With CLEM protein distributions visualized in fluorescence can be mapped onto the cellular ultrastructure measured with electron microscopy. Widespread application of correlative microscopy is hampered by elaborate experimental procedures related foremost to retrieving ...

Correlative Fluorescence- and Scanning, Transmission Electron Microscopy for Biomolecular Investigation

These days, cellular and molecular biology are closely integrated due to the development of novel correlative biomolecular microscopy platforms, even though this avant-garde vision of combining different imaging tools has been around since the late 1960s. The current boom and successful application of correlative imaging methods to dissect the cell’s internal structure is progressing faster tha...

Stochastic Micro-Pattern for Automated Correlative Fluorescence - Scanning Electron Microscopy

Studies of cellular surface features gain from correlative approaches, where live cell information acquired by fluorescence light microscopy is complemented by ultrastructural information from scanning electron micrographs. Current approaches to spatially align fluorescence images with scanning electron micrographs are technically challenging and often cost or time-intensive. Relying exclusivel...

Correlative Photoactivated Localization and Scanning Electron Microscopy

The ability to localize proteins precisely within subcellular space is crucial to understanding the functioning of biological systems. Recently, we described a protocol that correlates a precise map of fluorescent fusion proteins localized using three-dimensional super-resolution optical microscopy with the fine ultrastructural context of three-dimensional electron micrographs. While it achieve...

microscopy and analysis Correlative Light and Electron Microscopy